Xetra Bio Solution

Rabbit Polyclonal Aggrecan antibody. Suitable for IHC-P, WB and reacts with Rat, Human, samples. Cited in 166 publications. Alternative names Key facts Isotype IgG Host species Rabbit Storage buffer Standard buffer Clonality Polyclonal Immunogen The exact immunogen used to generate this antibody is proprietary information. Specificity This antibody detects recombinant human Aggrecan by Western Blotting. We have been unable to detect endogenous protein in WB with ab36861. Reactivity data IHC-P Tested Species Rat Dilution info 5 µg/mL Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. Species Human Dilution info 5 µg/mL Anti-Aggrecan antibody Aggrecan core protein, Cartilage-specific proteoglycan core protein, CSPCP, Agc, Agc1, Acan Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. WB Expected Species Rat, Human Dilution info Use at an assay dependent concentration. Notes - Notes Abcam is leading the way to address reproducibility in scientic research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use. If you do not nd a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specicity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals. Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specications meet your scientic needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.Product promise Tested We have tested this species and application combination and it works. It is covered by our product promise. Expected We have not tested this specic species and application combination in-house, but expect it will work. It is covered by our product promise. Predicted This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise. Not recommended We do not recommend this combination. It is not covered by our product promise.

Rabbit Polyclonal Collagen II antibody. Suitable for IP, ELISA, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Pig, Chicken, Human, Dog, Cow, Sheep samples. Cited in 767 publications. Immunogen corresponding to Full Length Protein corresponding to Human COL2A1
Alternative names

Collagen alpha-1(II) chain, Alpha-1 type II collagen, COL2A1

Key factsIsotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogens

• Full Length Protein corresponding to Human COL2A1. The exact immunogen used to generate this antibody is proprietary information. Database link P02458

Purification technique

Affinity purification Immunogen

Specificity

Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has expected cross-reactivity with Type IV and VI and negligible cross reactivity to Type I, III and V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.

ConcentrationConcentrations data is not available right now, please try again laterBatch concentration finder Purification notes

Collagen II Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens.

StorageShipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Add glycerol to a final volume of 50% for extra stability and aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Mouse Monoclonal VIP antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human VIP.
Alternative names Isotype

IgG2aForLiquidClonalityMonoclonalImmunogen  within Human VIP. The exact immunogen used to generate this antibody is proprietary information. Database link 

This antibody binds free Peptide Histidine Methionine in solution. It cross reacts with PACAP (approximately 12%, incomplete, non parallel inhibition). It does not cross react with GLP1, GLP2 VIP or glucagonLight chain type

 

Upon delivery aliquot

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Peptide Histidine Methionine (PHM) also known as PHM-27 is a bioactive peptide with a molecular mass of approximately 3 kDa. This peptide is primarily expressed in the gastrointestinal tract and the central nervous system where it is known for its role in neurotransmission and gut function. PHM operates by binding to specific receptors on the cell surface initiating a cascade of intracellular signaling events that contribute to its functional effects.

Biological function summary

Peptide Histidine Methionine impacts the regulation of water and salt intake as well as gastric secretion and motility. It does not function as part of a larger complex but acts individually to exert its effects. In the central nervous system it influences neurotransmission which can affect behaviors related to thirst and appetite. The peptide's ability to modulate these physiological processes highlights its potential significance in maintaining homeostasis.

Pathways

PHM interacts within the neuropeptide signaling pathway which regulates several bodily functions including fluid balance and stress responses. It also engages in pathways linked to the modulation of gastric acid secretion. PHM shares pathway relations with other peptides like vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating polypeptide (PACAP) which play complementary roles in physiological regulation.

Associated diseases and disorders

Peptide Histidine Methionine is associated with conditions such as irritable bowel syndrome (IBS) and dehydration disorders. Its role in the gastrointestinal tract links it to IBS where dysregulated secretion and motility are common issues. In the context of dehydration disorders PHM's involvement in fluid balance connects it to the regulation of body hydration. Studies have also suggested possible interactions with proteins like corticotropin-releasing hormone (CRH) that may further elucidate its role in these disorde

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

The Micro BCA Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions. This modification of the BCA Protein Assay is suitable for dilute protein samples (0.5-20µg/ml). The Micro BCA Protein Assay primarily relies on two reactions. Firstly, the peptide bonds in the protein sample reduce Cu2+ ions, in a temperature dependent reaction, from the copper solution to Cu+. The amount of Cu2+ reduced is proportional to the amount of protein present in the solution. Next, two molecules of bicinchoninic acid chelate with each Cu+ ion, forming a purple-colored product that strongly absorbs light at a wavelength of 562nm. The assay uses concentrated solutions and extended incubation times for the detection of dilute protein samples. The amount of protein present in a solution can be quantified by measuring the absorption spectra and comparing with protein solutions with known concentrations. The Micro BCA Protein Assay is suitable for quantifying protein solutions in 500 x 1ml assays or in >3,300 micro-well assays. The assay is supplied with a traditional bovine serum albumin (BSA) protein standard or a non animal protein standard.

Features

• Sensitive, colorimetric assay for dilute protein samples
• Linear range: 0.5-20µg/ml
• Compatible with most non-ionic and ionic detergents

Applications

• For dilute protein samples (0.5-20µg/ml)

For information on our regular Bicinchoninic Acid (BCA) Protein Assay, click here.  For samples with reducing agents, we recommend the Bicinchoninic Acid (BCA) Reducing Agent Compatible Protein Assay.

This kit is based on our GET™ Spin Columns, spin columns with a high binding affinity for DNA. GET™ AGAROSE DNA method involves release of nucleic acid fragments from gel pieces followed by capture of nucleic acids on the GET™ Spin Columns, washing, and elution of the clean nucleic acid fragments in a suitable buffer. The GET™ AGAROSE DNA  kits are supplied with enough reagents for 50 or 100 preps. Features Extract DNA fragments from agarose gels Rapid DNA isolation DNA ready for downstream applications, including ligations Suitable for 100-20,000bp fragments Compatible with TAE and TBE buffer gels Applications Isolation of DNA fragments from agarose gels. Cleaning and removing of contaminants from DNA samples

This kit is based on our GET™ Spin Columns, spin columns with a high binding affinity for DNA. GET™ AGAROSE DNA method involves release of nucleic acid fragments from gel pieces followed by capture of nucleic acids on the GET™ Spin Columns, washing, and elution of the clean nucleic acid fragments in a suitable buffer. The GET™ AGAROSE DNA  kits are supplied with enough reagents for 50 or 100 preps. Features Extract DNA fragments from agarose gels Rapid DNA isolation DNA ready for downstream applications, including ligations Suitable for 100-20,000bp fragments Compatible with TAE and TBE buffer gels Applications Isolation of DNA fragments from agarose gels. Cleaning and removing of contaminants from DNA samples

A comprehensive selection of reagents and kits for the purification, manipulation and analysis of DNA and RNA for molecular biology and cell biology studies.

Our genomic DNA purification kits purify high quality genomic DNA from a wide variety of sources and for a wide array of applications. OmniPrep™ genomic DNA kits are for ultra pure genomic DNA that is suitable for all downstream applications and are fully scalable. GET™ genomic DNA isolation kits are for the rapid isolation of DNA templates from small samples. It is based on genomic efficient technology that use solid phase for extraction, both spin columns and magnetic beads, XIT™ kits require no chloroform or phenol extraction and produce protein free, high quality DNA. OmniTemplate™ is designed for the rapid generation of PCR ready genomic templates. MegaLong™ genomic DNA extraction kits allow for DNA extraction from a variety of samples, including animal tissues, cultured cells, whole blood, bacteria and yeast or fungi.

A selection of products for plasmid DNA isolation, transformation and high throughput screening of plasmids can be found. Products include our unique and easy-to-use Plasmid Screening Toothpick™ and ToothPick™-PCR, which rapidly screens colonies for plasmid DNA, HP-Ampicillin™, which eliminates the problems of satellite or background colony formation and chemicals including Isopropyl β-D-1-thiogalactopyranoside (IPTG).

For transfection reagents, a series of four options are offered based on our G-FECT™ Transfection Reagent for every day transfections. The modified versions are designed for increased transfection efficiency and low cell toxicity.

For PCR (polymerase chain reaction), high quality and affordable reagents are offered, including Taq and Pfu Polymerases and dNTPs (Deoxynucleotides), indvidually or premixed.

For RT-qPCR (Reverse Transcriptase quantative Polymerase Chain Reaction), a wide selection of enzymes and kits are available.

For RNA research, products for the isolation of RNA, including Arrest™ Extraction Buffer, GET™ Total RNA, GET™ Total RNA-Mag and TriXtract™​, for the isolation of high quality RNA. Products for the detection and decontamination of RNase are available, including RNaseOUT™ that kills RNase on contact. 

For the detection of nucleic acids, agarose gel electrophoresis buffers and chemicals and DNA ladders are available and a proprietary nucleic acid assay that doesn’t require a spectrophotometer.

Numerous yeast research tools and accessories are also available to aid researchers in a wide variety of applications using yeast. A full range of molecular biology grade accessories, reagents and buffers for cell biology research are also available.

This kit is based on our GET™ Spin Columns, spin columns with a high binding affinity for DNA. GET™ AGAROSE DNA method involves release of nucleic acid fragments from gel pieces followed by capture of nucleic acids on the GET™ Spin Columns, washing, and elution of the clean nucleic acid fragments in a suitable buffer. The GET™ AGAROSE DNA  kits are supplied with enough reagents for 50 or 100 preps. Features Extract DNA fragments from agarose gels Rapid DNA isolation DNA ready for downstream applications, including ligations Suitable for 100-20,000bp fragments Compatible with TAE and TBE buffer gels Applications Isolation of DNA fragments from agarose gels. Cleaning and removing of contaminants from DNA samples